Toothbrushes are made of the shin bone of a cow (pure solid bone). Each one is the drilled by hand. Approx. 30 holes are drilled and each one is then filled with pure bristle. When completed, the back of the toothbrush is carefully painted with a matching enamel colour to disguise how these unique pieces are made. A wonderful miniature masterpiece that lasts a very long time as its pure bristle, and NOT nylon. Should outlast a normal toothbrush by 5 or 6 times. Always dry after cleaning with cold water on a soft towel.